Authors: Mitzi Rettinger, Uma Sreenivassan, Isil Dilek 
        Presented at Stevia World Americas 2010, Atlanta, GA, February 2010 
        
        
            Introduction
        
        
			FDA’s “no objection letters” in December 2008, opened the door to food and beverage companies to use Reb A (rebaudioside A) in food products as long as the purity is no less than 95%. Laboratories are now challenged with quantifying the percent of Reb A in the plant based material. For food and beverage manufacturers, it is not enough to receive a vendor’s certificate of analysis, it’s important to verify the quality and purity of the product. Reb A, a component of the Stevia leaf, has naturally occurring impurities that are variable depending on environmental conditions of farming, process of cultivation, harvest, and isolation/blending. Accuracy of this quantification depends on robustness of the analysis and quality of the reference. To accurately quantify the % Reb A (Assay) the lab must start with accurate reference standards.