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H-083 / H-083-1ML updated item number format

25-Hydroxyvitamin D3

100 ug/mL in Ethanol |

Certified Reference Material

A Certified Spiking Solution® of 25-Hydroxyvitamin D3, a prohormone whose blood serum levels are used to determine Vitamin D nutritional status. This solution standard is suitable for critical quantitative applications in research and clinical environments including: for use in preparation of controls and calibrators for LC/UV and LC-MS/MS assays; as system tuning/suitability standards; linearity verification; internal proficiency testing; and for OEM use in preparation of reagents, development of assays and instrument IQ/OQ. The stable, quantitative solution format provides significant benefits to the user including material savings, increased efficiency of labor, and convenience of use. The shelf stable Snap-N-Spike® format allows laboratories to eliminate painstaking weighing operations and provides the ability to spike a known concentration of analyte into their matrix of choice just before use.
Concentration: 100 ug/mL
Solvent: Ethanol
Unit Size: 1 mL/ampoule
CAS Number:


Chemical Formula:


Molecular Weight:


Certificate of Analysis:
US List Price:
$165.00 EA

E-086 / E-086-1ML    3-epi-25-Hydroxyvitamin D3, 50 μg/mL  
H-073 / H-073-1ML    25-Hydroxyvitamin D2, 50 μg/mL  
H-074 / H-074-1ML    D6-25-Hydroxyvitamin D3 (26,26,26,27,27,27-D6), 50 μg/mL  
H-089 / H-089-1ML    1α,25-Dihydroxyvitamin D3, 5 µg/mL  
H-090 / H-090-1ML    1α,25-Dihydroxyvitamin D2, 5 μg/mL  
H-107 / H-107-1ML    1,25-Dihydroxyvitamin D3-13C3 (25,26,27-13C3), 5 µg/mL  
V-024 / V-024-1ML    Vitamin D2, 1.0 mg/mL  
V-025 / V-025-1ML    Vitamin D3, 1.0 mg/mL  

Recommended Storage Condition
Ultra Freezer

UN Number, Class, PG
1170, 3, II

Safety Data Sheet

HS Code

▶  Accelerated Stability Studies by High Performance Liquid Chromatography | Greg Serna, Tamara Tarbox, Isil Dilek, Cerilliant, 2012
▶  Direct Quantitation of 1,25-(OH)2 Vitamin D3 in Serum Using a Highly Sensitive LC-MS/MS | Sha Joshua Ye; Changtong Hao; Mitesh Patel; Chuck Jolliffe; Frenny Ruparelia; Xuejun Zang; Javier Ramirez; Anil Oroskar; Asha Oroskar, IONICS Mass Spectrometry Group inc., 2014
▶  Evaluation of LCMS/MS scrambling ratios for deuterium-labeled Vitamin D metabolites, steroids and other compounds of clinical significance | Yunming Ying, Huahua Jian, Isil Dilek, Uma Sreenivasan, Cerilliant, 2011
▶  Evaluation of LCMSMS Deuterium Scrambling in Clinically Significant Small Molecules | Joshua Cooper, Isil Dilek, and Uma Sreenivasan, Cerilliant, 2012
▶  Fast and Accurate Analysis of Vitamin D Metabolites Using Novel Chromatographic Selectivity and Sample Preparation | Craig Aurand, David Bell, Anders Fridstrom, Klaus Buckendahl, Supelco, 2011
▶  LC/MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 on Ascentis® Express F5 | Sigma-Aldrich®, 2015
▶  LC/MS Analysis of 25-Hydroxyvitamin D2 and 25-Hydroxyvitamin D3 on Ascentis® Express F5 | Sigma-Aldrich, 2015
▶  LC/MS/MS Analysis of Vitamin D Metabolites on Ascentis® Express F5 | Sigma-Aldrich®, 2015
▶  LC/MS/MS of 25-Dihydroxyvitamin D2, 25-Hydroxyvitamin D3, and 3-epi-25-Hydroxyvitamin D3 in Serum or Plasma on Ascentis® Express F5 after SPE with HybridSPE®-Phospholipid, Minimization of Matrix Effects | Sigma-Aldrich®, 2015
▶  Method Optimization for LC-MS Analysis of Vitamin D Metabolite Critical Pairs in Serum Using HybridSPE®-Phospholipid, Ascentis® Express, and Other LC-MS Workflow Components | Supelco, 2014
▶  Mitigation of Deuterium Scrambling in Stable-Labeled Internal Standards during LC-MS/MS Analysis | Joshua Cooper, Billy Molloy, Huahua Jian, Lisa Calton, Derrell Johnson, Isil Dilek, Uma Sreenivasan, Don Cooper, Cerilliant, 2011

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