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Selection of Internal Standards for LC-MS/MS Applications

Authors: Uma Sreenivasan, Isil Dilek, Josh Cooper, Sarah Aijaz, Ravi Orugunty, Derrell Johnson, Heather Lima, Mitzi Rettinger
Presented at Mass Spectrometry Applications to the Clinical Lab (MSACL), San Diego, CA, March 28-April 1, 2015

Abstract
Internal standards (IS) are utilized across a wide range of mass spectrometry applications including therapeutic drug monitoring, newborn screening, endocrinology, and pain management testing. They are especially useful in improving the accuracy of quantitation in complex matrices. Internal standards work by normalizing for differences in extraction, injection, chromatography, ionization and detection between samples.

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