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High Quality, Certified Snap-N-Shoot Standards of Reb A & Stevia Impurities Ensure Accuracy & Consistency in Quantitative Applications

Authors: Mitzi Rettinger, Isil Dilek, PhD, Sandra Ybarra, Uma Sreenivasan, PhD, and Sherri Pogue
Presented at Association of Official Analytical Chemists (AOAC), Orlando, FL, September 2010

Abstract
FDA requirements for the use of Rebaudioside-A (Reb-A) in food and beverage products include a purity of no less than 95%. A component of the Stevia leaf, Reb-A contains naturally occurring impurities whose levels vary depending on the cultivation process, isolation and blending techniques, and the environmental conditions during farming and harvesting.

Laboratories often encounter multiple technical challenges in the analysis of Reb-A. The material properties of Reb-A provide significant issues with hygroscopicity, making preparation of a consistent and accurate reference standard problematic for laboratories. Weighing accuracy of mg amounts of Reb-A can be influenced by balance environment and weighing technique. Method selection may yield different results due to elution order differences between methods, issues with method accuracy or repeatability, or lack of impurity resolution. The use of an accurate, quantitative and stable solution standard of Reb-A and its impurities prepared in a controlled inert environment and packaged in an ampouled format under inert atmosphere eliminates these particular issues.

Multiple factors impact accuracy and consistency of a Reb-A reference standard. This poster will discuss these factors and their influence on the design, preparation, and certification of a solution standard of Reb-A and its impurities.


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